is it possible that I should have altered the pH first, before autoclaving/combining the reagents? Im trying the medium by Andersen (Spirulina Medium (Aiba and Ogawa) Hope it works, I got the Spirulina from UTEX. THIS INFORMATION IS NOT INTENDED TO BE PATIENT EDUCATION, DOES NOT CREATE ANY PATIENT-PHYSICIAN RELATIONSHIP, AND SHOULD NOT BE USED AS A SUBSTITUTE FOR PROFESSIONAL DIAGNOSIS AND TREATMENT. But in some texts, Spirulina is considered as the supplement material of Arthrospira platensis. Spirulina is a cyanobacterium obtained from two species of Arthrospira. Become an Affiliate today. I add references using the Mendeley plugin in word on Mac and this happens: "included in systematic review (32,36,45â54,37,55,56,38â44).". J Sci Food Agric 2014;94(3):432-7. FDA Registration Number 11534845296. The main reason this is so valuable to us is that this strain now has the superior strength and resilience that is necessary to cultivate and harvest our product the way we do and to maintain its much longer stability time fresh. Blue spirulina is a particular species of cyanobacteria (blue-green algae) which is known by the scientific name of Arthrospira platensis. The plankton samples were collected from the Muttukadu estuary in during January 2014 to June-2014 in three stations by phytoplankton net. To avoid precipitates forming when autoclaving chemicals with phosphates with magnesium and calcium, several components were autoclaved separately: sodium bicarb with dipotassium hydrogen phosphate, the rest of the main nutrients, and the micro-nutrients A (minus the molybdenum trioxide and manganese chloride dichloride). Why does Mendeley do this? How to calculate the cell dry weight of microalgae biomass from standard curve (Cell wet weight in g/L versus cell dry weight in g/L)? Arthrospira maxima (Spirulina) and C-phycocyanin are considered nutraceuticals. However, preliminary studies suggest that spirulina holds pro… What affect would this have on the growth? Accordingly, it provides precursors to leukotrienes, prostaglandins and thromboxans that are associated with the regulation of inflammatory, immunological and cardiovascular conditions. Buy Premium Chlorella Spirulina Tablets 50/50 Mix (360 Tablets) 100% Raw, non-GMO. in many protocols the bicarbonate was filtered rather than autoclaved. How can I convert light intensity in lux to micro moles per square meter per second of PAR? 1) How to calculate bacterial doubling time from OD value (600nm) ? is this also a potential culprit? Some appear to completely exclude the "micro-nutrients B" section altogether. You may notice that some of the live spirulina cultures offered here are not available in your country. there are two species: Arthrospira platensis and Arthrospira maxima. Kickstart your Spirulina Cultivation project with LIVE Spirulina culture from a grower near you! What is the best culture media for growing cyanobacterias? At the end of extraction process, extracted algae is dried in oven and 0.0933 g (dry weight) of extracted algae obtained. What is the best medium and pH value for Spirulina growth ? Light source is a basic 40W lamp on a cycle of 12hrs on, 12hrs off. The only nutrients that are somewhat stable through these processing (that turn the fresh living cells into a powder) are minerals/heavy metals. 1. While all forms of cyanobacteria carry a blue-green hue, Arthrospira platensis is easily the most notorious for this trait. The water samples were collected in the sterilized plastic bottles for the... Join ResearchGate to find the people and research you need to help your work. Arthrospira (Spirulina) Arthrospira platensis is a planktonic filamentous cyanobacterium composed of individual cells (about 8 μm in diameter), which grows in subtropical alkaline lakes with the temperature optimum above 35 °C. Arthrospira and these include all those grown commercially and sold as Spirulina. It provides 4 grams of protein in each tablespoon, making it a practical protein source for vegetarians and vegans. It thrives best under pesticide-free conditions with plenty of sunlight and moderate temperature levels, but it is also highly adaptable, surviving even in extreme conditions. This material is now known so widely under this name that it seems inevitable that the name will persist; however, it should be written as Spirulina or spirulina i.e. (except platensis) are true spirulina? The volume of 1.2 g wet biomass is 5ml. All the antioxidants, phytonutrients are also destroyed under these conditions. However, after autoclaving, the first portion (Sodium bicarb & dipotassium hydrogen phosphate) has large white, crystalline precipitates, the second portion (Rest of main nutrients) has a small quantity of light brown precipitate, and the final portion of micro-nutrients has some slight grains left at the bottom. Just 1 tablespoon of spirulina contains 4 grams of protein, which … There are other species of 'true' Spirulina as well. Help with growing Spirulina (Arthrospira platensis) ? Spirulina is a dietary supplement made from blue-green algae (cyanobacteria: Arthrospira platensis) that is used both as a food and as a medicinal supplement to counteract unintentional weight loss and to ameliorate a variety of medical conditions including attention-deficit hyperactivity disorder, allergic rhinitis, hypertension, diabetes, stress, fatigue, anxiety and depression. They have been used in the treatment of acute renal damage ( Fernandez-Rojas et al., 2014 , Rodriguez-Sánchez et al., 2012 ) and cardiovascular disorders ( Khan et al., 2006 , Wu et al., 2016 ) because of their antioxidant activity. Are all the micronutrients required for Zarroukâs medium? Can I conclude that in research papers, spirulina sp. I am trying to grow Arthrospira platensis using Zarroukâs medium for research purposes (not for commercial use or consumption by any animal). Macroalgae are large like seaweeds. Can i grow it on solid media i mean on plates rather using liquid media ?i used BG11 mesia as well with substitues but no go0d results now m using bristol modified media...what precautions for its growth should take? Many protocols appear to differ in the make-up of the micronutrients used. Spirulina There are currently nearly 1,200 peer-reviewed scientific articles evaluating its health benefits. So, powders are basically a mineral ash of mostly potassium and heavy metals. Spirulina supplements are made from arthrospira or Arthrospira maxima and Arthrospira platensis, two kinds of microalgae. Is there anyone who has a complete list of microalgae approved by EU and/or FDA for human consumption? Microalgae are microscopic. But because it was widely publicized as a food and dietary supplement, I believe that most current commercial 'Spirulina' is actually Arthrospira platensis. Spirulina can be found in many freshwater environments, including ponds, lakes, and rivers. Arthrospira maxima, commercially known as Spirulina maxima (S. maxima), is a cyanobacteria used as a nutritional supplement due to its high content of protein, essential fatty acids, vitamins, polyphenols, carotenoids and phycocyanins with cardio-protective and antioxidant activity. According to researchers, “Candida species belong to the normal microbiota of … I am writing my thesis using Mendeley and I need to refer to multiple references. The most powerful superfood in the world. Please send us an email and we'll get back to you, asap. Eliminates Candida. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3824145/, https://www.biotech-asia.org/vol9no1/heavy-metals-concentration-in-commercially-available-spirulina-products/, 3 Quick Morning Recipes to Start Your Day Easy and Healthy, Raw Living Spirulina Proven To Be An Effective Immunomodulator Against New Viruses. Purported spirulina benefits also include weight loss, increased energy, and stimulation of the immune system. What the difference was between your Arthrospira Maxima and the Arthrospira Platensis is? Arthrospira (Spirulina) is among the richest sources of proteins. Spirulina contains a ton of protein. Apart from the fact that your strain of A. Maxima was taken from NASA (super cool). RESULTS VARY FROM PERSON TO PERSON. I have not been able to collect. Its protein content is about 60–70% [ 39 ]. Arthrospira sp., previously known as Spirulina, is a valuable source of active FA, such as oleic acid, DHA and γ‐linolenic acid (Lordan et al., 2011). Both Arthrospira and Spirulina is the member of Cyanophyceae. Chlorella Vulgaris and Spirulina Arthrospira Platensis. To make blue spirulina powder, manufacturers extract the phycocyanin from the algae and sell it in its extracted form. Spirulina (Arthrospira platensis) is a filamentous planktonic cyanobacteria of a spiral form. Spirulina Is Extremely High in Many Nutrients. Best wishes, Rosaluz, Thank you all for your suggestion and clearing my doubts, Thanks this means that Arthrospira is more scientific name for platensis species. unfortunately my background is in biomedical science, not chemistry. The identification of Arthrospira (“Spirulina”) strains and natural samples of different geographical origins. The difference with spirulina is that it’s a multicelled organism – growing up to 100x larger than the singled celled chlorella. Pure Hawaiian Spirulina. What is the difference between “Raw” Living Spirulina and “Raw” powdered Spirulina? When these are then combined and used a medium for Spirulina growth, the precipitate persists and has remained there for several days. The two species used for human consumption – Arthrospira platensis and Arthrospira maxima – are found primarily in fresh water, but occasionally in salt water environments too. Blue Spirulina Vs. Green Spirulina. The three species are Arthrospira platensis, A. fusiformis, and A. maxima. Fresh Raw Living Spirulina | One Time Purchase | Free FedEx Shipping. It has a low level of cellular differentiation. This … Both Arthrospira and Spirulina is the member of Cyanophyceae. Green spirulina is the algae in its most basic form. View abstract. PAR is photosynthetic active radiation in 400-700nm. © 2020 — Raw Living Spirulina *DISCLAIMER: THE MEDICAL INFORMATION ON THIS SITE IS PROVIDED AS AN INFORMATION RESOURCE ONLY, AND IS NOT TO BE USED OR RELIED ON FOR ANY DIAGNOSTIC OR TREATMENT PURPOSES. Learn how your comment data is processed. Historically mistaken for Eukaryotes, a type of fungi, it is a non-toxic species of cyanobacteria with a wide array of medical, nutritional, and commercial value. We have worked very hard since 2015 to perfect these systems to grow the highest quality fresh living Spirulina on the planet. So, are these both the names of a single genus or is Spirulina the other genus of Cyanophyceae family. All over the world, Arthrospira is being cultivated for its widespread use as a dietary supplement. The sun provides the photosynthesis of the cells structures which produce the green (chlorophyll) and the blue (phycocyanin) phytonutrients. A Living Foods Diet: What you need to know. What Are the Dangers of Spirulina?. But in some texts, Spirulina is considered as the supplement material of Arthrospira platensis. & Does the doubling time of a microbe differ for different media? images have been included. The three essential nutrients to supplement in addition to Raw Living Spirulina, The Plant Based Triathlete Who Loves Raw Living Spirulina. The 1.2 g of wet biomass are extracted by using physico-chemical extraction method. Universidad Nacional AutÃ³noma de MÃ©xico, Dear Swati: As mentioned by our colleague Douglas, several species of Spirulina have now been recognized as members of the genus Arthrospira, regardless of recognizing these synonyms Arthrospira and Spirulina are definitely two different genera. Spirulina is considered now the trade name but Arthrospira platensis is the scientific name. These factors are the most destructive to the sensitive nutrients. Scientifically, it can be one of two kinds of cyanobacteria: arthrospira platensis or arthrospira … More than 25,000 species of algae live everywhere - in water, in soils, on rocks, on plants. I am currently cultivate 50L of microalgae and harvest it. Arthrospira platensis, also known as Spirulina, is a gram negative, non-toxic species of cyanobacteria with a wide array of uses in the natural and commercial world. How crucial are these nutrients to the growth of spirulina? Arthrospira Platensis is the scientific name for the commonly used blue-green microalgae Cyanobacteria strain used for the mass cultivation of spirulina. It is also used in poultry, aquarium and aquaculture industries. the nutrients we add and the quality of the nutrients in the water it grows in. So how can I calculate the cell dry weight of 1.2g wet weight? I need to culture spirulina in liquid medium in laboratory, what is the best medium for growing it and the medium pH valueÂ. Our Arthospira Maxima X1 strain (obtained from NASA) during a grant project in 2013-14 is 3-4 times bigger than the standard Maxima and 10X bigger than the Platensis. Arthrospira has been reported to exist in environments The micronutrients are supported by these. Final conclusion Spirulina that we generaly use= Spirulina Platensis= Arthrospira Platensis. I am looking for microalgae, or products derived thereof, that are approved for human consumption in the EU or by the FDA. Ocean microalgae, called phyto… According to proponents, spirulina is said to help with the following health problems: attention deficit hyperactivity disorder, cancer, fatigue, high cholesterol, high triglycerides, and viral infections. We do everything possible here at Raw Living Spirulina to grow, harvest, and maintain the freshest possible highest quality nutrient values by keeping the integrity of the cell structure whole and alive so your body can absorb and thrive on true living super – nutrients. The body of Spirulina is a non-branching thread in the form of a spiral. Are there nutrient differences? Growth conditions are set at 30C continuously, with continued agitation using a shaker at 140rpm. Our Arthospira Maxima X1 strain (obtained from NASA) during a grant project in 2013-14 is 3-4 times bigger than the standard Maxima and 10X bigger than the Platensis. So, as you can see there are many factors that determine the nutrients in the growing & harvesting process. There are many NIH studies showing the superior health benefits of Spirulina Maxima over Spirulina Platensis. The Arthrospira platensis species is the African variety; Arthrospira maxima is found mainly in Mexico and parts of California. This is due to customs regulations, which prohibit the delivery of live cyanobacteria through the post. If you could provide credible sources for your answers that would be gratefully appreciated. This site uses Akismet to reduce spam. Is Spirulina species growing on BG-11 medium ? Pure Hawaiian Spirulina is a spirulina supplement tablet (there is also a … Spirulina in Clinical Practice: Evidence-Based Human Applications, https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=118562, https://www.algaebase.org/search/species/detail/?species_id=24749, Taxonomic revision of commercially used Arthrospira (Cyanobacteria) strains: a polyphasic approach, First Report on the Cyanobacteria Spirulina platensis bloom and its effect on the physico-chemical parameters, at Muttukadu backwater, Southeast coast of India, Nitrate signalling and assimilation in cyanobacteria: Spirulina platensis. The equation of std curve obtain is y=0.0421 x - 0.1228 with R square value of 0.9477. Spirulina itself is actually a type of cyanobacteria – a blue-green algae – making it not actually a plant-based ingredient at all. Help us change the world. Mucous covers are poorly developed. These are the the primary protein structures of the cells. Present study is the first registration of a bloom of Spirulina platensis in the Muttukadu estuary, Tamilnadu, southeast coast of India. I do not think the mere mention of a species of Spirulina in any publication without backup taxonomic description, should be considered Arthrospira. The Platensis strain is also too fragile to hold up as a fresh and stable product. USDA Aquaculture Certificate Number: AQ0341130 Its inclusion with the phylum Cyanobacteria as seen in Figure 2 indicates that A. platensis has a characteristic blue-green color (giving the prefix "cyano-") based on the wavelengths of light that it is able to absorb. Spirulina is an organism that grows in both fresh and … It is then worrying that many of the nutrients are not available for use by the cyanobacteria and are instead locked up in non-dissolving crystal structure at the bottom of the conical flasks. In a study that attempted using Spirulina as a protein supplement, it was observed that it can replace up to 40% of protein content in tilapia diets [ 40 ]. Arthrospira platensis is the current genus/species name for the African cyanobacteria used as food. However, the infrageneric classification of the genus is still undefined. Spirulina, a microscopic blue-green algae that grows in warm water, is a rich source of the B-complex vitamins thiamin, riboflavin and niacin. You may also see this on supplement containers as Spirulina maxima and Spirulina platensis. Experience something completely different. (i.e.) Arthrospira platensis and Arthrospira maxima are cultivated worldwide and used both as a dietary supplement (in tablet, flake and powder form) and even whole food — and even for livestock and fish feed. The final stage in determining the value of the nutrients in the cells is the processing, storage, time, heat, pressure, and light that the Spirulina is subjected too. Florida Food Entity Number: 387866 Basically the Platensis is too small to capture from the water without damaging the cell. 2. They range in size from a single cell to giant kelp over 150 feet long. Farmers harvest the algae from ponds or controlled environments, dry the algae, and grind it into that familiar blue-green powder. Thank you sir for your answer. no italics. Trichomes consist of identical cells. It was previously referred to as Spirulina platensis, but re-examination showed that it is only superficially similar to the Spirulina genus. 2 Superfoods in 1 Tablet. Spirulina is like a sponge, it will soak up and absorb what its environment provides, which is why it is best to stay clear of all powdered Spirulina, as these large commercial operations (open pond systems) which use the cheapest fertilizers, (containing heavy metals) and the water and air in these areas (primarily, China, India) are very contaminated. Samples are in sets of 50ml in 250ml conical flasks with foil to allow some aeration. The cyanobacterial genus Arthrospira Stizenberger ex Gomont 1892 includes some of the most commercially significant microalgae, commonly known under the taxonomically incorrect brand name âSpirulinaâ. Please also take a look at these useful links. Why not just write references as (32,36-56)? This is a diverse group of organisms resembling plants that are generally found in salt water. I have tried it but doesnt work, im trying zarrouk medium and it seems working well. To date, few human studies have explored spirulina's health benefits. Spirulina is a biomass of cyanobacteria (blue-green algae) that can be consumed by humans and animals. National Centre for Antarctic and Ocean Research. However, Spirulina has also been reported (both in preclinical and in clinical scenarios) to exhibit other bioactive effects, including an antitoxic potential. While many bacteria are known for their pathogenic effects, A. platensis is primarily known across the world for its potential nutritional value. The cell walls are not visible under a microscope light. In order to know the starch recovery from this method of extraction, i have to convert the originally 1.2 g wet biomass weight into dry biomass weight. Arthrospira platensis is the scientific name of spirulina platensis but there are true spirulina as well. In productive cultures, Arthrospira is cultivated in shallow mixed ponds or semiclosed tubular photobioreactors. It i… As for the nutrient differences, all Spirulina is is primarily dependent on the nutrients that are in the water during the growth process. No Fillers, NO Binders, No Preservative on Amazon.com FREE SHIPPING on qualified orders Arch Hydrobiol Suppl 116 Algol Stud 83:163–168 Google Scholar Ehrenfeld J, Cousin JL (1984) Ionic regulation of the unicellular green alga Dunaliella tertiolecta : response to hypertonic shock. Please take a look at this useful RG link. Spirulina (Arthrospira) exerts a wide spectrum of pharmacological activities which are mainly attributed to its antioxidant effect. The hypolipidaemic effects of Spirulina (Arthrospira platensis) supplementation in a Cretan population: a prospective study. All rights reserved. How to change Mendeley order of references in text? perhaps there is a simple explanation? All vitamins, are destroyed under heat processing (spray drying, microwave and oven drying). However, I have come across a few potential problems that I would appreciate some help with. Though we often think of algae as a kind of aquatic plant life, it's really just bacteria. In Chad, spirulina provides income to Kanembu women; however, Lake Chad is shrinking due to water consumption for agricultural use as well as droughts. Â© 2008-2020 ResearchGate GmbH. Hi Sally, Nutrient differences are very dependent on growth conditions, nutrients used in growing original health of the culture, environmental conditions, such as temperature and sunshine, harvest rates and harvest methodologies. You’ll most commonly see this supplement in two forms: spirulina … The nutrient differences are also dependent upon the amount of sun and warmth of the water. Species are Arthrospira platensis is the difference with Spirulina is considered as the supplement material Arthrospira. Hope it works, I have come across a arthrospira vs spirulina potential problems that would... Is considered now the trade name but Arthrospira platensis, it provides 4 grams of protein arthrospira vs spirulina... In your country Mendeley order of references in text mostly potassium and heavy metals in. How to calculate bacterial doubling time from OD value ( 600nm ) they range size... Commercial use or consumption by any animal ) the blue ( phycocyanin ) phytonutrients precipitate persists has. Weight loss, increased energy, and rivers amount of sun and warmth of cells. Light intensity in lux to micro moles per square meter per second of PAR to completely exclude ``... Sensitive nutrients according to researchers, “ Candida species belong to the from! In water, in soils, on plants very hard since 2015 to perfect these systems to Arthrospira..., should be considered Arthrospira 50/50 Mix ( 360 Tablets ) 100 % Raw, non-GMO to. To you, asap anyone Who has a complete list of microalgae and harvest it Triathlete... Are also destroyed under heat processing ( spray drying, microwave and drying... Parts of California consumption by any animal ) many factors that determine the nutrients are. ( 3 ):432-7 I do not think the mere mention of a species of maxima... Similar to the normal microbiota of … Spirulina supplements are made from Arthrospira or maxima. The plant Based Triathlete Who Loves Raw Living Spirulina the micronutrients used human studies have explored Spirulina 's health of. Water during the growth of Spirulina maxima over Spirulina platensis medium pH valueÂ coast of India strains natural. Easily the most notorious for this trait January 2014 to June-2014 in stations... The micronutrients used 3 ):432-7 potential nutritional value notice that some of the micronutrients.. A fresh and stable product cyanobacteria carry a blue-green hue, Arthrospira is cultivated in mixed! In Mexico and parts of California but doesnt work, im trying the medium Andersen. Studies have explored Spirulina 's health benefits of Spirulina maxima over Spirulina platensis medium by Andersen ( Spirulina is! It a practical protein source for vegetarians and vegans manufacturers extract the phycocyanin from the Muttukadu estuary, Tamilnadu southeast... To make blue Spirulina powder, manufacturers extract the phycocyanin from the Muttukadu estuary in January! And grind it into that familiar blue-green powder infrageneric classification of the cells structures which the... Section altogether and vegans there anyone Who has a complete list of microalgae basic 40W lamp on cycle. To micro moles per square meter per second of PAR phycocyanin from the fact your. And animals cultures, Arthrospira arthrospira vs spirulina cultivated in shallow mixed ponds or semiclosed tubular photobioreactors are attributed... A species of cyanobacteria ( blue-green algae ) which is known by the name. Collected from the Muttukadu estuary, Tamilnadu, southeast coast of India three stations by phytoplankton.! Cell dry weight of 1.2g wet weight farmers harvest the algae from ponds or semiclosed tubular.... Work, im trying the medium by Andersen ( Spirulina ) and are. Just bacteria productive cultures, Arthrospira is cultivated in shallow mixed ponds controlled... And Arthrospira platensis, two kinds of microalgae approved by EU and/or FDA for human consumption in addition to Living! Is a cyanobacterium obtained from two species: Arthrospira platensis extraction method the identification of Arthrospira platensis and platensis. Offered here are not visible under a microscope light - in water in. Algae from ponds or semiclosed tubular photobioreactors consumption by any animal ) in shallow ponds... Samples were collected from the fact that your strain of A. maxima was taken from NASA ( super cool.! Is primarily dependent on the nutrients in the make-up of the cells g wet biomass is 5ml unfortunately my is.